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CLIA: Laboratory Requirements

The Clinical Laboratory Improvement Amendments (CLIA) regulations establish quality standards for all laboratory testing performed on human specimens to ensure accuracy, reliability, and timeliness. Any facility performing laboratory testing on human specimens for health assessment, diagnosis, prevention, or treatment must have a CLIA certificate.

Citation: 42 C.F.R. Part 493
Sections: 220
Words indexed: 71,550
Applies to: Any facility that performs laboratory testing on human specimens for health purposes, including hospitals, reference labs, physician offices, nursing facilities, pharmacies, and clinics

Key Points

All labs testing human specimens must be CLIA-certified, including physician office labs and point-of-care testing sites

Four certificate types: waived, provider-performed microscopy, moderate complexity, and high complexity

Waived tests (e.g., rapid strep, urine dipstick, glucose monitoring) have minimal regulatory requirements

Moderate and high complexity labs must meet personnel, QC, proficiency testing, and quality assessment standards

Labs must enroll in an approved proficiency testing program for each regulated analyte

Key Areas

Certification

Certificate types, application, inspection

Personnel

Director qualifications, testing personnel requirements

Quality Systems

QC, proficiency testing, quality assessment

Key Provisions

493.15

Certificate Types

Determines which certificate a lab needs based on test complexity. This drives all subsequent regulatory requirements.

493.1251

Quality Control (QC)

Requirements for control procedures, including calibration, control testing frequency, and corrective actions for out-of-range results.

All Regulation Sections

Part 493CLIA: Laboratory Requirements(220)